Design and adjustment of coupled microalgae oil extraction methods for the development of a topology of biorefinery

Ángel Darío González-Delgado
Viatcheslav Kafarov



Microalgae have the potential to produce a big amount of biodiesel per area unit owing to its high lipid content. However, for a sustainable biodiesel production from microalgae, the concept of biorefinery must be taken into account; this concept can be applied to microalgae biomass for the production of biofuels and high added value products owing to different components present in microalgae strains. Microalgae oil extraction is an important step to take into account for the development of a topology of biorefinery. The main objective of this work is to establish different coupled methods for cell disruption and oil extraction of bioprospected microalgae. Three methods were designed and adjusted to local conditions. For cell disruption experiments, effect of autoclave, hydrolysis and organosolv pretreatment on the increase of extraction efficiency was evaluated. For oil extraction, three solvent based methodologies were developed, ethanol/hexane mixture, continuous reflux solvent extraction and solvent extraction with high speed homogenization. Microalgae used in experimental development were Closterium sp., Navicula sp., Amphiprora sp., Guinardia sp., Botryococcus sp., Desmodesmus sp. and Tetraselmis sp. microalgae strains. Cell disruption procedures that presents better results were acid hydrolysis and organosolv pretreatment, best results for continuous reflux solvent extraction were reached when extraction time was 8 hours and hexane is used as extraction solvent, while solvent extraction with high speed homogenization shows higher oil yields when are used times around 14 minutes and frequencies of 5000 rpm.

Texto completo:



. Chisti, Y., Biodiesel from microalgae, Biotechnology Advances,

Vol. 25, pp 294–306, 2007.

. González-Delgado, A.D., Kafarov, V., Microalgae

based biorefinery: Issues to consider. CT&F - Ciencia, Tecnología

y Futuro, Vol. 4, No 4, pp 5-22, 2011.

. González, A.D., Kafarov, V., Guzman, A. Development

of a methodology of microalgae oil extraction in the

biodiesel from microalgae production chain (In Spanish),

Prospectiva, Vol. 7, No 2, pp 53 – 60, 2009.

Prospect. Vol. 10, No. 1, Enero - Junio de 2012, págs. 113-123

. Bligh, E. G. & Dyer, W. J., A rapid method of total lipid

extraction and purification. Canadian Journal of Biochemistry

and Physiology, Vol. 37, pp 911–917, 1959.

. Ramirez Fajardo, A., Esteban Cerdan, L., Robles Medina,

A., Gonzales Moreno, P., Molina Grima, E., Lipid

extraction from the microalga Phaeodactylum tricornutum,

European Journal of Lipid Science and Technology, Vol. 109,

No 2, pp 120–126, 2007.

. Halim, R., Gladman, B., Danquah, M., Webley, P., Oil

extraction from microalgae for biodiesel production, Bioresource

Technology, Vol. 102, No. 1, pp 178-185, 2011.

. Peñaranda, L.A., Sepulveda, K., Alvarez, Y.A.,

González-Delgado, A.D., Kafarov, V., Evaluation of lipid

and monosaccharide obtaining routes of microalgae biomass

under the biorefinery concept (In Spanish). ION, Vol.

, No 2, pp 13-22, 2011.

. González, A., Kafarov, V., Design of a multifunctional

reactor for third generation biofuels production. Chemical

Engineering Transactions, Vol. 21, pp 1297-1302, 2010.

. González, A.D., Amaya, A., Sarmiento R., Kafarov, V., Desarrollo

de una metodología para la extracción de aceite de

microalgas empleando disrupción celular térmica y química.

Book of abstracts. XXIX Latin American Chemistry Congress –

CLAQ 2010. ISBN 978-958-99607-0-7, S. N. FQT 64, 2010.

. Córdoba, L.S., López, L.M., González, A.D., Kafarov,

V., Obtención de aceite de microalgas nativas a escala de

laboratorio mediante la combinación de disrupción celular

y método de extracción soxhlet para la producción de

biodiesel. Book of abstracts. IV International Congress of Biofuels

Science and Technology CIBSCOL 2010. ISBN: 978-958-

-8185-6, pp 61, 2010.

. González, S., Galindo, L., González, A. D., Kafarov V.,

Adaptación del método Bligh & Dyer a la extracción de lípidos

de microalgas nativas para la producción de biodiesel.

Book of abstracts. XXIX Latin American Chemistry Congress –

CLAQ 2010. ISBN 978-958-99607-0-7, S. N. MYE 18, 2010.

. Cravotto, G., Biffa, L., Mantegna, S., Pergo, P., Avogadro,

M., Cintas, P., Improved extraction of vegetable

oils under high-intensity ultrasound and/or microwaves.

Ultrasonics Sonochemistry. Vol. 15, No. 5 pp 898-902, 2008.

Enlaces refback

  • No hay ningún enlace refback.

Licencia Creative Commons
Este trabajo esta licenciado bajo una Licencia Internacional Creative Commons Atribución-NoComercial-SinDerivados 4.0.


ISSN : 1692-8261 Versión impresión
ISSN : 2216-1368 Versión Web

RedesRepositorio UACRedes Sociales

Licenciada bajo:

Indexada en: