Morphologycal response and multiparameter comparison of microalgae oil extraction alternatives for obtaining biodiesel and co-products./Respuesta morfológica y comparación multiparámetro de alternativas de extracción de aceite de microalgas

Ángel Darío González Delgado



Microalgae biomass emerges as an energy crop of great interest worldwide owing to its potential for obtaining biofuels and high value products and for the development of biorefinery processes owing to their varied composition and high biomass productivities per area unit. Several alternatives of microalgal metabolites extraction and transformation are being studied for the development of novel technologies for biomass using. Microalgae oil extraction is particularly important because is a key stage in microalgal biodiesel production chains and their efficiency contributes significantly to global process efficiency. In this study, a comparison of five oil extraction methods in lab-scale, taking into account extraction efficiency, energy consumption, costs of extraction and toxicity was made using five national bioprospecting microalgae strains. Methods evaluated were Solvent extraction with high speed homogenization (SHE), Continuous efflux solvent extraction (SCE), Hexane based extraction (HBE), Cyclohexane based extraction (CBE) and Ethanol-hexane extraction (EHE), microalgae strains used were Closterium sp., Amphiprora sp., Navicula sp., Nannochloropsis sp., and Guinardia sp., morphologycal response of strains to oil extraction methods was also evaluated using optic microscopy. Results shows that although there is not an unique oil extraction method with the best performance in all evaluated criteria, SCE, SHE and HBE methods presents high oil yields, while HBE method is the more convenient in global terms for use in lab scale and appears as an emerging technology for microalgae oil extraction in large scale.

Palabras clave

Microalgas; Extracción; Eficiencia;Costos;Requerimientos energéticos; Toxicidad;

Texto completo:



Singh. PN., Singh, A., Production of liquid biofuels from renewable resources, Progr Energ Combust Sci, 37(1), 52-68, 2011.20

Chisti, Y., A bioeconomy vision of sustainability, Biofuels Bioprod Biorefin, 4, 359–61, 2010.

Lam, MK., Lee, KT., Microalgae biofuels: A critical review of issues, problems and the way forward, Biotechnol Adv., 30(3), 673-90, 2012.

Khoo, HH., Sharratt, PN., Das, P., Balasubramanian, RK., Naraharisetti, PK., Shaik, S., Life cycle energy and CO2 analysis of microalgae-to-biodiesel: Preliminary results and comparisons, Bioresour Technol., 102, 5800-07, 2011.

UIS-ICP-Morrosquillo, Bioprospecting of Colombian microalgae for biodiesel production, Final Report, Bucaramanga, 2011.

Cherubini, F., The biorefinery concept: using biomass instead of oil for producing energy and chemicals, Energy Convers Manage, 51, 1412–21, 2010.

González-Delgado, AD., Kafarov, V., Microalgae based biorefinery: Issues to consider, CT&F Cienc Tecnol Future, 4(4), 5-21, 2011.

Singh, J., Gu, S., Commercialization potential of microalgae for biofuels production, Renew Sustain Energy Rev, 14, 2596–610, 2010.

González, A., Kafarov, V., Design of a multifunctional reactor for third generation biofuels production, Chem Eng Transact, 21, 1297-302, 2010.

Henriques, M., Silva, A., Rocha, J., Extraction and quantification of pigments from a marine: A simple and reproducible method, Commun Curr Res Educ Top Trends Appl Microbiol, 1, 586-93, 2007.

Peñaranda, LA., Sepúlveda, KJ., Álvarez, YE., González-Delgado, AD., Kafarov, V., Evaluation of lipid and monosaccharide obtaining routes of microalgae biomass under the biorefinery concept, Ion, 24(2), 13-22. 2011.

Wilkie, A., Mulbry, W., Recovery of dairy manure nutrients by benthic freshwater algae, Bioresour Technol, 84(1), 81-91, 2002.

Harun, R., Singh, M., Forde, G., Danquah, M., Bioprocess engineering of microalgae to produce a variety of consumer products, Renew Sust Energ Rev, 14, 1037–47, 2010.

Mussgnug, J., Klassen, V., Schlüter, A., Kruse, O., Microalgae as substrates for fermentative biogas production in a combined biorefinery concept, J Biotechnol., 150(1), 51-6, 2010.

González, AD., Kafarov, V., Guzmán, A., Development of a methodology of microalgae oil extraction in the biodiesel from microalgae production chain, Prospectiva, 7, 53–60, 2009.

González, AD., Kafarov, V., Design and adjustment of coupled methods of microalgae oil extraction for third generation biofuels production in a topology of biorefinery, Prospectiva, 10(1), 113-123, 2012.

Córdoba, LS., López, LM., González, AD., Kafarov, V., Diseño de una metodología para la extracción de aceite de microalgas nativas mediante el método soxhlet, Book of abstracts, XXIX Latin American Chemistry Congress –CLAQ 2010, ISBN 978-958-99607-0-7: S. N. MYE 113.

Fajardo, AR., Cerdan, LE., Medina, AR., Fernandez, FGA., Moreno, PAG., Grima, EM., Lipid extraction from the microalga Phaeodactylum tricornutum, Eur J Lipid Sci Technol 2007, 109, 120–6, 2010.

Sarmiento, R., Amaya, A., González, AD., Kafarov, V., Desarrollo de una metodología para la extracción de aceite de microalgas empleando disrupción celular térmica y química, Book of abstracts. XXIX Latin American Chemistry Congress – CLAQ 2010. ISBN 978-958-99607-0-7: S. N. FQT 64. 2010.

Galindo, L., González, S., González, AD., Kafarov, V., Adaptation of the Bligh & Dyer method to the extraction of lipids of native microalgae for biodiesel production, Book

of abstracts, XXIX Latin American Chemistry Congress – CLAQ. ISBN 978-958-99607-0-7 2010: S. N. MYE 18. 2010.

García, J., Miranda, J., González, AD., Kafarov, V., Comparison of microalgae oil extraction methods for biodiesel production, Book of abstracts, IV International Congress of Biofuels Science and Technology CIBSCOL:81. 2010.

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