Evaluación citotóxica y biocompatibilidad de la aleación AZ31B para aplicaciones en ingeniería de tejidos óseos / Cytotoxic evaluation and biocompatibility of AZ31B alloy for applications in bone tissue engineering.

Authors

  • Kevin Daniel Genez Valencia National University of Colombia image/svg+xml
  • Viviana Marcela Posada Pérez National University of Colombia image/svg+xml
  • Gloria Patricia Fernández Morales Pontifical Bolivarian University image/svg+xml
  • Juan Fernando Ramírez Patiño National University of Colombia image/svg+xml

DOI:

https://doi.org/10.15665/rp.v14i2.691

Keywords:

Citotoxicidad, Aleaciones de Magnesio, Biocompatibilidad, Metales Biodegradables, Regeneración Ósea, Metales Celulares, Espumas Metálicas

Abstract

El desarrollo de materiales innovadores para la fabricación de implantes óseos biodegradables es uno de las tópicos más importantes de investigación en el área de los biomateriales. Los materiales a partir de los cuales se fabrican estos implantes, pueden ser gradualmente disueltos, absorbidos, consumidos o excretados por el cuerpo humano, lo que elimina la necesidad de procedimientos quirúrgicos secundarios para la remoción del implante. Las aleaciones de magnesio (Mg) aparecen como materiales promisorios para ser usados en aplicaciones ortopédicas, teniendo en cuenta su potencial biocompatible. Sin embargo, por sus propiedades químicas, este material se corroe rápidamente, produciendo burbujas de gas subcutáneas lo que puede resultar en productos de corrosión tóxicos. De ahí que el uso del Mg y sus aleaciones como material biodegradable para aplicaciones ortopédicas no está completamente establecido. Por estas razones, el objetivo de este trabajo es determinar los efectos citotóxicos de los productos de corrosión de la aleación AZ31B usando la configuración de espuma metálica fabricada a partir de dicho material.

Author Biographies

  • Kevin Daniel Genez Valencia, National University of Colombia

    Estudiante Maestría en Ingeniería Mecánica, Facultad de Minas, Grupo de Investigación en Biomecánica e Ingeniería de la Rehabilitación (GI-BIR), Universidad Nacional de Colombia, Medellín, Colombia.

  • Viviana Marcela Posada Pérez, National University of Colombia

    Estudiante Doctorado en Ingeniería – Ciencia y Tecnología de Materiales, Facultad de Minas, Grupo de Investigación en Biomecánica e Ingeniería de la Rehabilitación (GI-BIR), Universidad Nacional de Colombia, Medellín, Colombia.

  • Gloria Patricia Fernández Morales, Pontifical Bolivarian University

    Doctora en Ingeniería, Facultad de Ingeniería Industrial, Grupo de Investigación en Nuevos Materiales (GINUMA), Universidad Pontificia Bolivariana, Medellín, Colombia.

  • Juan Fernando Ramírez Patiño, National University of Colombia

    Doctor en Ingeniería, Facultad de Minas, Grupo de Investigación en Biomecánica e Ingeniería de la Rehabilitación (GI-BIR), Universidad Nacional de Colombia, Medellín, Colombia.

References

H. S. Brar, J. Wong, and M. V. Manuel, “Investigation of the mechanical and degradation properties of Mg-Sr and Mg-Zn-Sr alloys for use as potential biodegradable implant materials,” J. Mech. Behav. Biomed. Mater., vol. 7, pp. 87–95, 2012.

T. Adachi, Y. Osako, M. Tanaka, M. Hojo, and S. J. Hollister, “Framework for optimal design of porous scaffold microstructure by computational simulation of bone regeneration,” Biomaterials, vol. 27, no. 21, pp. 3964–3972, 2006.

N. Li and Y. Zheng, “Novel Magnesium Alloys Developed for Biomedical Application: A Review,” J. Mater. Sci. Technol., vol. 29, no. 6, pp. 489–502, 2013.

G. Song, “Control of biodegradation of biocompatable magnesium alloys,” Corros. Sci., vol. 49, no. 4, pp. 1696–1701, 2007.

L. Wu, B. J. C. Luthringer, F. Feyerabend, A. F. Schilling, and R. Willumeit, “Effects of extracellular magnesium on the differentiation and function of human osteoclasts,” Acta Biomater., vol. 10, no. 6, pp. 2843–2854, 2014.

L. Wu, F. Feyerabend, A. F. Schilling, R. Willumeit, and B. J. C. Luthringer, “Effects of extracellular magnesium extract on the proliferation and differentiation of human osteoblasts and osteoclasts in coculture,” Acta Biomater., vol. 27, pp. 294–304, 2015.

F. Witte, F. Feyerabend, P. Maier, J. Fischer, M. Störmer, C. Blawert, W. Dietzel, and N. Hort, “Biodegradable magnesium-hydroxyapatite metal matrix composites,” Biomaterials, vol. 28, no. 13, pp. 2163–2174, 2007.

L. Li, J. Gao, and Y. Wang, “Evaluation of cyto-toxicity and corrosion behavior of alkali-heat-treated magnesium in simulated body fluid,” Surf. Coatings Technol., vol. 185, pp. 92–98, 2004.

J. Fischer, D. Pröfrock, N. Hort, R. Willumeit, and F. Feyerabend, “Reprint of: Improved cytotoxicity testing of magnesium materials,” Mater. Sci. Eng. B Solid-State Mater. Adv. Technol., vol. 176, no. 11, pp. 1773–1777, 2011.

J. Trinidad, I. Marco, G. Arruebarrena, J. Wendt, D. Letzig, E. Sáenz De Argandoña, and R. Goodall, “Processing of magnesium porous structures by infiltration casting for biomedical applications,” Adv. Eng. Mater., vol. 16, no. 2, pp. 241–247, 2014.

X. N. Gu, W. R. Zhou, Y. F. Zheng, Y. Liu, and Y. X. Li, “Degradation and cytotoxicity of lotus-type porous pure magnesium as potential tissue engineering scaffold material,” Mater. Lett., vol. 64, pp. 1871–1874, 2010.

B. Lina, “Evaluación del Potencial Bioactivo de las Esponjas Marinas Myrmekioderma gyroderma y Topsentia ophiraphidites,” pp. 1–35, 2012.

M. P. Staiger, A. M. Pietak, J. Huadmai, and G. Dias, “Magnesium and its alloys as orthopedic biomaterials: A review,” Biomaterials, vol. 27, pp. 1728–1734, 2006.

X. Gu, Y. Zheng, Y. Cheng, S. Zhong, and T. Xi, “In vitro corrosion and biocompatibility of binary magnesium alloys,” Biomaterials, vol. 30, no. 4, pp. 484–498, 2009.

R. M. Lozano, B. T. Pérez-Maceda, M. Carboneras, E. Onofre-Bustamante, M. C. García-Alonso, and M. L. Escudero, “Response of MC3T3-E1 osteoblasts, L929 fibroblasts, and J774 macrophages to fluoride surface-modified AZ31 magnesium alloy,” J. Biomed. Mater. Res. - Part A, vol. 101, no. 10, pp. 2753–2762, 2013.

L. Yang, N. Hort, D. Laipple, D. Höche, Y. Huang, K. U. Kainer, R. Willumeit, and F. Feyerabend, “Element distribution in the corrosion layer and cytotoxicity of alloy Mg-10Dy during in vitro biodegradation,” Acta Biomater., vol. 9, no. 10, pp. 8475–8487, 2013.

Y. F. Zheng, X. N. Gu, and F. Witte, “Biodegradable metals,” Mater. Sci. Eng. R Reports, vol. 77, pp. 1–34, 2014.

Y. Ding, C. Wen, P. Hodgson, and Y. Li, “Effects of alloying elements on the corrosion behavior and biocompatibility of biodegradable magnesium alloys: a review,” J. Mater. Chem. B, vol. 2, no. 14, pp. 1912–1933, 2014.

H. R. B. Rad, M. H. Idris, M. R. A. Kadir, and S. Farahany, “Microstructure analysis and corrosion behavior of biodegradable Mg-Ca implant alloys,” Mater. Des., vol. 33, no. 1, pp. 88–97, 2012.

S. Zhang, X. Zhang, C. Zhao, J. Li, Y. Song, C. Xie, H. Tao, Y. Zhang, Y. He, Y. Jiang, and Y. Bian, “Research on an Mg-Zn alloy as a degradable biomaterial,” Acta Biomater., vol. 6, no. 2, pp. 626–640, Feb. 2010.

F. R. A. J. Rose and R. O. C. Oreffo, “Bone Tissue Engineering: Hope vs Hype,” Biochem. Biophys. Res. Commun., vol. 292, no. 1, pp. 1–7, 2002.

Downloads

Additional Files

Published

2016-06-16

Issue

Vol. 14 No. 2 (2016): Julio - Diciembre

Section

Articles

License

The authors to publish in this journal agree to the following conditions:

  1. The authors transfer the copyright and give the the journal first publication right of the work registered with Creative Commons Attribution License, which allows third parties to use the published work on the condition of always mentioning the authorship and first publication in this journal.
  2. The authors may perform other independent and additional contractual arrangements for the non-exclusive distribution of the version of the article published in this issue (E.g., Inclusion in an institutional repository or publication in a book), it must be indicated clearly that the work was first published in this journal.
  3. It allows and encourages the authors to publish their work online (eg institutional or personal pages) before and during the review and publication process. It can lead to productive exchanges and greater and faster dissemination of the published work (see The Effect of Open Access)

Instructions to fill out Certificate of Originality and Copyright Assignment

  1. Click here and get the forms of Certificate of Originality and Copyright Assignment  .
  2. In each field to fill out, click and complete the corresponding information.
  1. Once the fields are filled out, at the end of the form copy your scanned signature or digital signature. Please adjust the size of the signature on the form.
  2. Finally, you can save them as pdf files and send them through the OJS platform as an attachment.