Potencial biotecnológico de levaduras identificadas en Norte de Santander
DOI:
https://doi.org/10.15665/rp.v20i1.2729Abstract
Yeasts are eukaryotic organisms called unicellular fungi, they present oval, spherical, cylindrical, or ellipsoidal morphology. This group has been exploited in various industrial fields and the agri-food sector, specifically in the production of beers, bread and wine. Yeast isolates obtained in different environments of the Norte de Santander department, are kept in the Strain Bank of the Francisco de Paula Santander University. In order to characterize and identify these microorganisms, the strains were recovered and purified, their morphology was described macroscopically and microscopically; they were identified using the API 20 C AUX biochemical gallery; viability tests were carried out on different conservation methods, and finally stored in the strain bank. According to the results, the 25 strains obtained correspond to: Candida guilliermondii (9), Saccharomyces cerevisiae (5), Candida tropicalis (4), Candida famata (2), Candida utili (1), Cryptococcus laurentii (1), Cryptococcus albidus (1), Trichosporon mucoides (1), and Candida pelliculosa (1). It was established that the 30% glycerol preservation method is ideal for yeasts. The strains can be used in research and explore their biotechnological potential in different productive sectors. The strains can be used in research and explore their biotechnological potential in different productive sectors. The genera: Candida, Cryptococcus and Trichosporon are of clinical consideration, which should be handled with caution.
References
A. Díaz, A. Villareal, S. De los Santos, F. I. Parra, A. Sepúlveda, and M. Yadira, "Potencial biotecnológico y ambiental de los microorganismos," La sociedad académica, pp. 52-63, 2017. https://www.researchgate.net/publication/330370877_Potencial_biotecnologico_y_ambiental_de_los_microorganismos
N. M. Montaño, A. L. Sandoval , S. L. Camargo, and J. M. Sánchez, "Los microorganismos: pequenos gigantes," Elementos, vol. 77, p. 10, 2010. http://redalyc.uaemex.mx/src/inicio/ArtPdfRed.jsp?iCve=29411989003
G. I. Estrada y M. C. Ramírez, Micología General. Manizales - Caldas: Cárol Castaño Trujillo, 2019. http://www.ucm.edu.co/wp-content/uploads/libros/Micologia_general.pdf
J. A. Cuevas, "Los Hongos: Héroes y villanos de la prosperidad humana " Revista digital universitaria, vol. 17 (9), p. 10, 2016. http://www.revista.unam.mx/vol.17/num9/art69/
J. A. Vásquez, M. Castrillón, and Z. I. Monsalve, "Actualización en caracterización molecular de levaduras de interés industrial," Revista Colombiana de Biotecnología, vol. 18, p. 12, 2016. http://www.redalyc.org/articulo.oa?id=77649147015
M. Quirós, V. Rojas, R. Gonzalez, y P. Morales, "Selection of non-Saccharomyces yeast strains for reducing alcohol levels in wine by sugar respiration," International Journal of Food Microbiology, vol. 181, pp. 85-91, 2014. https://www.sciencedirect.com/science/article/pii/S0168160514002001
F. E. Argote, R. A. Cuervo, E. Osorio, J. Delgado-Ospina, and H. S. Villada, "Evaluation of ethanol production from molasses with native strains Saccharomyces cerevisiae.," Biotecnología en el Sector Agropecuario y Agroindustrial, vol. 13, pp. 40-48, 2015. https://www.cabdirect.org/cabdirect/abstract/20163073454
C. Machín, N. Antonio, G. Carralero, C. Amarilys, and G. Rodríguez, "Levadura Saccharomyces cerevisiae y la producción de alcohol.," Revista ICIDCA, vol. 50, pp. 20-29, 2016. https://www.researchgate.net/publication/313899904_LEVADURA_SACCHAROMYCES_CEREVISIAE_Y_LA_PRODUCCION_DE_ALCOHOL_Revision_bibliografica_YEAST_SACCHAROMYCES_CEREVISIAE_AND_THE_PRODUCTION_OF_ALCOHOL_A_Review
L. A. Rubio, "Evaluación del grado de contaminación microbiana con streptococcus mutans y streptococcus sanguis en fresas de diamante, posterior a la preparación cavitaria clase I según Black, previamente autoclavadas," Odontología, Facultad de odontología Univesidad central del Ecuador, Quito 2015. http://www.dspace.uce.edu.ec/bitstream/25000/4563/1/T-UCE-0015-157.pdf
L. P. Bermeo, D. M. Flórez, M. Ocampo, and M. Franco, "Colección de cepas microbianas usadas en formación y proyectos de investigación aplicada en el centro para la formación cafetera SENA Regional Caldas," Revista Nova, vol. 2, pp. 8-15, 2016. http://revistas.sena.edu.co/index.php/rnova/article/view/615
M. Linares and F. Solís, "Identificación de levaduras," Revista Iberoamericana de Micología p. 20, 2015. http://www.guia.reviberoammicol.com/Capitulo11.pdf
L. B. Lopez, "Actinomicetos aislados del suelo del Jardín botánico de la Universidad Tecnológica de Pereira," Scientia et technica, vol. 19, pp. 223-229, 2014. https://revistas.utp.edu.co/index.php/revistaciencia/article/view/8397
A. Sosa, N. González, Y. García, Y. Marrero, E. Valiño, J. Galindo, et al., "Collection of microorganisms with potential as additives for animal nutrition at the Institute of Animal Science," Cuban Journal of Agricultural Science, vol. 51, p. 10, 2017. https://www.researchgate.net/publication/324418819_Collection_of_microorganisms_with_potential_as_additives_for_animal_nutrition_at_the_Institute_of_Animal_Science
K. L. Vaquera, A. G. Tapia, A. Hernández, I. E. P. Guerrero, y J. C. T. Guzmán, "Optimización de un medio de cultivo para la producción de levaduras con interés biotecnológico," Jóvenes En La Ciencia, vol. 4, p. 6, 2018. http://148.214.90.90/index.php/jovenesenlaciencia/article/view/2608
C. R. Barranco, L. A. Pérez, N. P. Alfaro, and J. Y. Escorcia, "Evaluación de medios de cultivo naturales para el mantenimiento de Colletotrichum sp. y Saccharomyces cerevisiae," Scientia et Technica, vol. 23, pp. 397-404, 2018. https://dialnet.unirioja.es/servlet/articulo?codigo=6760213
A. Bonifaz, F. Montelongo, J. Araiza, G. M. González, R. Treviño, A. Flores, et al., "Evaluación de MALDI-TOF MS para la identificación de levaduras patógenas oportunistas de muestras clínicas," Revista chilena de infectología, vol. 36, pp. 790-793, 2019. https://scielo.conicyt.cl/scielo.php?pid=S0716-10182019000600790&script=sci_arttext&tlng=en
M. Alvarez, F. Tucta, E. Quispe, and V. Meza, "Incidencia de la inoculación de microorganismos benéficos en el cultivo de fresa (Fragaria sp.)," Scientia Agropecuaria, vol. 9, pp. 33-42, 2018. http://www.scielo.org.pe/scielo.php?script=sci_arttext&pid=S2077-99172018000100004
P. L. Castillo, C. A. Betancur, and E. Pardo, "Caracterización de microorganismos con potencial probiótico aislados de estiércol de terneros Brahman en Sucre, Colombia," Revista de Investigaciones Veterinarias del Perú, vol. 29, pp. 438-448, 2018. http://www.scielo.org.pe/scielo.php?pid=S1609-91172018000200006&script=sci_arttext
L. C. Trujillo and S. E. Hernández, "Aislamiento y caracterización de levaduras presentes en el fruto del Syzygium malaccense (l.) Merr. &L. M Perry (pomorroso) en la comuna 1 de la ciudad de Neiva-Huila," Ingeniería y Región, vol. 13, p. 10, 2015. https://journalusco.edu.co/index.php/iregion/article/view/707
N. Morales and N. Castro, "Métodos de diagnóstico en micología," Revista CES Medicina, vol. 32, p. 12, 2018. https://dialnet.unirioja.es/servlet/articulo?codigo=6337244
T. Ortiz, V. Ocampo, L. D. Prada, and M. Franco, "Métodos de conservación para actinobacterias con actividad solubilizadora de fósforo," Revista Colombiana de Biotecnología, vol. 18, p. 8, 2016. http://www.scielo.org.co/scielo.php?pid=S0123-34752016000200005&script=sci_abstract&tlng=en
A. A. Sánchez and P. Sibaja, "Peritonitis terciaria por Candida famata: infección por una levadura atípica," Acta Médica Costarricense, vol. 59, p. 3, 2017. https://www.scielo.sa.cr/scielo.php?pid=S0001-60022017000100035&script=sci_arttext
J. Pineda, T. Uribarren, and L. R. Castañón, "Candidosis vaginal: Revisión de la literatura y situación de México y otros países latinoamericanos," Revista Médica de Risaralda, vol. 23, pp. 38-44, 2017. https://pdfs.semanticscholar.org/e808/69f895075b351bab6b7cbdd3fd0af3d5a8a0.pdf
K. López, K. R. Dzul, C. Lugo, J. J. Arias, and J. E. Zavala, "Mecanismos de resistencia antifúngica de los azoles en Candida albicans," Revista biomédica, vol. 27, pp. 127-136, 2016. https://www.medigraphic.com/cgi-bin/new/resumen.cgi?IDARTICULO=67960
V. Lazo, G. Hernández, and R. Méndez, "Candidiasis sistémica en pacientes críticos, factores predictores de riesgo," Horizonte Médico (Lima), vol. 18, pp. 75-85, 2018. http://www.scielo.org.pe/scielo.php?pid=S1727-558X2018000100011&script=sci_arttext
S. Zurita, "Situación de la resistencia antifúngica de especies del género Candida en Perú," Revista Peruana de Medicina Experimental y Salud Publica, vol. 35, p. 6, 2018. http://www.scielo.org.pe/scielo.php?pid=S1726-46342018000100019&script=sci_arttext&tlng=pt
G. Mariel, "Identificación fenotípica de Candida dubliniensis aislada de candidosis de mucosa oral en pacientes inmunodeprimidos," Dermatología, vol. 63, p. 144, 2019. https://smdac.org.mx/wp-content/uploads/2019/02/dermaENE-FEB2019completo-ca-o-1.pdf#page=31
G. Rizzitelli, E. Guanziroli, A. Moschin, R. Sangalli, and S. Veraldi, "Onychomycosis caused by Trichosporon mucoides," International Journal of Infectious Diseases, vol. 42, pp. 61-63, 2016. https://www.ijidonline.com/article/S1201-9712(15)00272-6/fulltext
A. R. d. Magalhães, M. M. Nishikawa, S. S. B. d. Mondino, H. W. d. Macedo, E. M. d. S. d. Rocha, and A. R. d. S. Baptista, "Trichosporon isolation from human ungueal infections: is there a pathogenic role?," Anais brasileiros de dermatologia, vol. 91, pp. 173-179, 2016. http://dx.doi.org/10.1590/abd1806-4841.20164632
Y. J. Choe, D. B. Blatt, A. Yalcindag, S. F. Geffert, A. M. Bobenchik, and I. C. Michelow, "Cryptococcus albidus Fungemia in an Immunosuppressed Child: Case Report and Systematic Literature Review," Journal of the Pediatric Infectious Diseases Society, vol. 9, pp. 100-105, 2019. https://doi.org/10.1093/jpids/piz039
J. Mejía and A. Saavedra, Conociendo las levaduras. Saber Mas. 2006. https://www.sabermas.umich.mx/archivo/articulos/97-numero-131/193-conociendo-las-levaduras.html
O. L. Ostos, S. M. Rosas, y J. L. González, "Aplicaciones biotecnológicas de los microorganismos," Nova, vol. 17, p. 35, 2019. https://revistas.unicolmayor.edu.co/index.php/nova/article/view/950
M. Díaz de los Ríos, G. Saura, y I. Pérez, "La producción de levadura Candida Utilis (Levadura Torula)," in Resultados de ICIDCA, ed Cuba, pp. 338-351, 2019. https://www.researchgate.net/publication/337170496La_produccion_de_levadura_Candida_Utilis_Levadura_Torula
C. Rosales, K. Valerín, y V. Jiménez, "Crecimiento dimórfico y caracterización molecular de Candida guillermondi aislado de Pannicum maximun," Revista Tecnología en Marcha, vol. 31, p. 11, 2018. https://www.scielo.sa.cr/scielo.php?pid=S0379-39822018000100120&script=sci_arttext
K. Manjarres, M. Arias, Y. A. Molina, M. I. Betancur, y E. Rodríguez, "Producción de xilitol por Candida guilliermondii a partir de fermentación de residuos de palma de aceite xylitol production by Candida guilliermondii from fermentation of waste oil palm.," Revista U.D.C.A. Actualidad & Divulgación Científica, vol. 19(2), p. 7, 2016. https://www.udca.edu.co/wp-content/uploads/2018/01/revista-udca-actualidad-divulgacion-cientifica-2016-2.pdf#page=151
C. Martínez "Hongos microscópicos del néctar floral," Farmacia Proyecto bibliográfico, Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Sevilla, España, 2019. https://hdl.handle.net/11441/92014
C. Y. Portillo, "Identificación de levaduras presentes en el proceso de transformación de polen corbicular a pan de abejas por métodos tradicionales y moleculares," Magister en Producción Animal Investigación Departamento de Ciencias para la Producción Animal, Universidad Nacional de Colombia, Bogotá, 2016. https://repositorio.unal.edu.co/handle/unal/56727
G. Fornari, V. A. Vicente, R. R. Gomes, M. D. Muro, R. L. Pinheiro, C. Ferrari, et al., "Susceptibility and molecular characterization of Candida species from patients with vulvovaginitis," Brazilian Journal of Microbiology, vol. 47, pp. 373-380, 2016. https://www.sciencedirect.com/science/article/pii/S1517838216000526
O. I. Indralaya, "Identification of Candida species by assimilation and Multiplex-PCR methods," Journal of Chemical Technology and Metallurgy, vol. 52, p. 8, 2017. https://www.researchgate.net/publication/321126992
U. M. Malik, A. Khan, and M. L. Satti, "Comparative evaluation of CHROMagar and API 20C AUX in isolation and identification of Candida species," Journal of Islamic International Medical College, vol. 13, pp. 85-90, 2018. https://jiimc.riphah.edu.pk/wp-content/uploads/2018/07/Uzma-Mussarat-Malik.pdf
V. Reis, A. Bassi, J. Silva, and S. Ceccato, "Characteristics of Saccharomyces cerevisiae yeasts exhibiting rough colonies and pseudohyphal morphology with respect to alcoholic fermentation," Brazilian Journal of Microbiology, vol. 44, p. 11, 2013. http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400014&nrm=iso
B. T. Tadesse, A. B. Abera, A. T. Tefera, D. Muleta, Z. T. Alemu, and G. Wessel, "Molecular Characterization of Fermenting Yeast Species from Fermented Teff Dough during Preparation of Injera Using ITS DNA Sequence," International journal of food science, vol. 2019, p. 8, 2019. https://www.hindawi.com/journals/ijfs/2019/1291863/
R. Pekka, L. Jake, C. S. Adrian, T. Zhihao, S. Saija, K. Aleksi, et al., "Quantitative analysis of colony morphology in yeast," BioTechniques, vol. 56, pp. 18-27, 2014. https://www.future-science.com/doi/abs/10.2144/000114123
V. L. Belini, H. Suhr, and P. Wiedemann, "Online monitoring of the morphology of an industrial sugarcane biofuel yeast strain via in situ microscopy," Journal of Microbiological Methods, vol. 175, p. 105973, 2020. http://www.sciencedirect.com/science/article/pii/S0167701220302785
S. Zhang, Q. Zhao, W. Xue, Y. Li, Y. Guo, X. Wu, et al., "The Isolation and Identification of Candida Glabrata from Avian Species and a Study of the Antibacterial Activities of Chinese Herbal Medicine In-Vitro," Poultry Science, p. 38, 2021. https://www.sciencedirect.com/science/article/pii/S0032579121000377
E. Casalone, C. Barberio, L. Cappellini, and M. Polsinelli, "Characterization of Saccharomyces cerevisiae natural populations for pseudohyphal growth and colony morphology," Research in Microbiology, vol. 156, pp. 191-200, 2005. https://www.sciencedirect.com/science/article/pii/S0923250804002578
T. Cortes, P. López, and J. Cuervo, Criobiología: Criopreservación de microorganismos y de otras fuentes biológicas a bajas temperaturas., 2016. https://www.researchgate.net/publication/299560675_Criobiologia_Criopreservacion_de_microorganismos_y_de_otras_fuentes_biologicas_a_bajas_temperaturas
O. E. Ladino, J. David, and C. A. Chacin, "Evaluación de dos métodos de conservación de hongos filamentosos patógenos de palma de aceite," Centro agrícola, vol. 43, pp. 36-41, 2016. http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-57852016000200005
S. Huertas, M. Casas, M. Morales, Z. Moreno, and D. Castaño, "Implementación y evaluación de dos métodos de conservación y generación de la base de datos del banco de cepas y genes del Instituto de Biotecnología de la Universidad Nacional de Colombia (IBUN)," Nova, vol. 4, p. 11, 2006. https://revistas.unicolmayor.edu.co/index.php/nova/article/view/55
Y. Ocares and J. F. Castro, "Preservación de microorganismos por congelación," in Boletin INIA, I. d. I. Agropecuarias, Ed., ed Santiago de Chile, p. 18, 2020. https://biblioteca.inia.cl/handle/123456789/67167
M. Guerra and J. F. Castro, "Evaluación de viabilidad de microorganismos," in Boletin INIA I. d. I. Agropecuarias, Ed., ed Santiago de Chile, p. 17, 2020. https://biblioteca.inia.cl/handle/123456789/67168
Downloads
Published
Issue
Section
License
Copyright (c) 2022 Liliana Yaneth Suarez, Alba Luz Rangel Riaño
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The authors to publish in this journal agree to the following conditions:
- The authors transfer the copyright and give the the journal first publication right of the work registered with Creative Commons Attribution License, which allows third parties to use the published work on the condition of always mentioning the authorship and first publication in this journal.
- The authors may perform other independent and additional contractual arrangements for the non-exclusive distribution of the version of the article published in this issue (E.g., Inclusion in an institutional repository or publication in a book), it must be indicated clearly that the work was first published in this journal.
- It allows and encourages the authors to publish their work online (eg institutional or personal pages) before and during the review and publication process. It can lead to productive exchanges and greater and faster dissemination of the published work (see The Effect of Open Access)
Instructions to fill out Certificate of Originality and Copyright Assignment
- Click here and get the forms of Certificate of Originality and Copyright Assignment .
- In each field to fill out, click and complete the corresponding information.
- Once the fields are filled out, at the end of the form copy your scanned signature or digital signature. Please adjust the size of the signature on the form.
- Finally, you can save them as pdf files and send them through the OJS platform as an attachment.
