Controlled incubation system for the growth of Aspergillus flavus with intelligent control and visual monitoring in LabVIEW
DOI:
https://doi.org/10.15665/rp.v24i1.3901Keywords:
Aspergillus flavus, classic control, intelligent control, biological incubation, temperature control, visual monitoring, LabVIEWAbstract
This article presents the design and implementation of a controlled incubation system for the growth of Aspergillus flavus, aimed at supporting experimental studies under regulated environmental conditions. The system incorporates a temperature sensor (PT1000) and humidity sensor (DHT22), a thermal conditioning module based on Peltier cells, and an ultrasonic humidifier; integrated through a control architecture that combines classical and intelligent techniques. A dynamic model of the thermal loop was obtained through parametric identification, enabling the evaluation of PID controllers tuned using the Ziegler–Nichols method, an optimal I-PD configuration, and a fuzzy PD controller. Experimental results show that the I-PD controller provides the best performance, achieving 0% overshoot, a steady-state error of 0.005 °C, and robustness against disturbances. The physical prototype maintained stable operating conditions between 25–37 °C and relative humidity above 85%, with a maximum deviation of 0.2 °C during 10 hours of continuous operation. Additionally, a real-time visual monitoring module was integrated to document fungal growth through RGB channel separation, enabling non-invasive observation of morphological development. The results confirm the feasibility of the system for controlled fungal incubation studies.
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