Green synthesis of graphene oxides and comparative evaluation of their cytotoxicity according to the oxidation degree against pancreatic cancer cells
DOI:
https://doi.org/10.15665/m15p2691Keywords:
Graphene oxide, eco-friendly synthesis, green synthesis, oxidation degrees, pancreatic cancerAbstract
Graphene oxide (GO) was synthesized using alternative methods aimed at reducing the use of traditionally employed toxic reagents such as H₂O₂ and H₂SO₄, to minimize environmental impact and improve the biocompatibility of the material. The synthesis was optimized by controlling variables such as temperature and reaction time, allowing the degree of GO oxidation to be adjusted. FTIR spectra exhibited characteristic bands of oxygenated groups, including carbonyl, hydroxyl, epoxy, and single carbon-oxygen bonds (C=O// O-C-O/ C-OH // C-O), confirming effective control of the oxidation level above 70%. The modification of the process allowed a reduction in the cytotoxicity of GO for healthy Vero cells (high cell viability), unlike PC-3 neoplastic cells, which had low cell viability with values close to or less than 60%. This material is well-suited for potential biomedical applications, including tissue engineering, controlled drug delivery, and the development of antimicrobial surfaces. The results obtained validate the effectiveness of the proposed approaches, highlighting the importance of implementing sustainable and safe synthesis routes to enhance the properties of GO in the field of nanotechnology applied to health.
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